. 2017 Dec 5;3(4):dvx019. doi: 10.1093/eep/dvx019

Table 4:

Functional annotation clustering (FAC) as performed in DAVID

Annotation category	Term	Enrichment score	Gene	FlyBase gene ID	Function	P-value of ANOVA	Significance of ANOVA
INTERPRO	Immunoglobulin-like domain/fold	11.42	mesh	FBgn0051004	Ig fold	0.000611	***
INTERPRO	Immunoglobulin-like domain/fold	11.42	Ppn	FBgn0003137	Ig domain, extracellular matrix structural constituent	0.0101	*
INTERPRO/GO_TERM_BP_DIRECT/GOTERM_MF_DIRECT	Proteolysis, peptidase	6.16	Jon65Ai	FBgn0035667	Serine-type endopeptidase, proteolysis	0.00293	**
			MP1	FBgn0027930	Serine-type endopeptidase, proteolysis	0.00187	**
			thetaTry	FBgn0011555	Digestive enzyme with serine-type peptidase activity	1.68E-08	***
			zetaTry	FBgn0011556	Digestive enzyme with serine-type peptidase activity	0.000488	***
			Decay	FBgn0028381	Cysteine-type endopeptidase activity, caspase	0.00188	**
			Damm	FBgn0033659	Cysteine-type endopeptidase activity, caspase	6.79E-06	***
			alphaTry	FBgn0003863	Serine-type endopeptidase, proteolysis	5.69E-05	***
			Ser7	FBgn0019929	Serine-type endopeptidase, proteolysis	1.51E-12	***
			Ser6	FBgn0011834	Serine-type endopeptidase, proteolysis	5.17E-03	**
			Swim	FBgn0034709	Polysaccharide binding, cysteine-type peptidase activity, proteolysis	1.35E-02	*
			Jon74E	FBgn0023197	Serine-type endopeptidase, proteolysis	1.58E-03	**
			psh	FBgn0030926	Peptidase and serine-type endopeptidase activity, defense response	4.41E-08	***
			Jon65Aiv	FBgn0250815	Serine-type endopeptidase, proteolysis	1.74E-04	***
			Jon65Aiii	FBgn0035665	Serine-type endopeptidase, proteolysis	6.77E-05	***
			Ance-4	FBgn0033366	Peptidase	6.97E-04	***
			Ance	FBgn0012037	peptidyl-dipeptidase activity	6.97E-04	***
			Jon25Bii	FBgn0031654	Serine-type endopeptidase, proteolysis	8.62E-04	***
GOERM_BP_DIRECT/INTERPRO/GOTERM_MF_DIRECT	G-protein-coupled receptor signalling, rhodopsin-like	6.02	Rh3	FBgn0003249	G-protein coupled photoreceptor activity	3.73E-03	**
			AkhR	FBgn0025595	Ccarbohydrate, lipid and tryglyceride homeostasis	1.88E-09	***
			Rh2	FBgn0003248	G-protein coupled photoreceptor activity	4.65E-02	*
			Galphas	FBgn0001123	Signal transduction, chemical synaptic transmission	1.07E-11	***
			ninaE	FBgn0002940	G-protein coupled photoreceptor activity, rhodopsin-like	2.80E-05	***
			Ggamma1	FBgn0004921	G-protein, gamma subunit, signal transducer activity	4.16E-05	***
			Gbeta76C	FBgn0004623	G-protein coupled photoreceptor activity	2.71E-02	*
			Rh4	FBgn0003250	G-protein coupled photoreceptor activity	1.83E-03	**
GOTERM_MF_DIRECT/GOTERM_BP_DIRECT	Fatty acid elongation	3.49	eloF	FBgn0037762	Fatty acid elongase	4.60E-05	***
GOTERM_MF_DIRECT	Neuropeptide hormone activity/binding	3.35	Nplp2	FBgn0040813	Neuropeptide signaling, humoral immune response	1.39E-02	*
			Nplp3	FBgn0042201	Neuropeptide hormone activity, neuropeptide signaling	3.45E-01	NS
			Neb-cGP	FBgn0083167	Hormone activity, regulation of growth	3.72E-02	*
GOTERM_MF/CC/BP_DIRECT	Calcium-dependent phospholipid binding	2.72	AnxB9	FBgn0000083	calcium ion binding, calcium-dependent phospholipid binding	7.69E-03	**
GPTERM_MP_DIRECT, INTERPRO, COG_ONTOLOGY	Calcium ion binding, signal transduction, EF-hand domain	2.5	Cals	FBgn0039928	Calcium ion binding, chemical synaptic transmission	2.29E-02	*
			Gel	FBgn0010225	Actin binding, calcium ion binding	1.52E-03	**
			Scp2	FBgn0020907	Calcium ion binding	3.43E-02	*
			Scp1	FBgn0020908	Calcium ion binding	8.74E-02	.
			Mlc2	FBgn0002773	Calcium ion binding	3.33E-04	***
			Zasp52	FBgn0265991	Muscle development	3.91E-08	***
			LpR1	FBgn0066101	Uptake of neutral lipids from circulation, calcium ion binding	4.93E-01	NS
			Mlc1	FBgn0002772	Calcium ion binding, myosin	3.91E-04	***
			up	FBgn0004169	Calcium ion binding, calcium ion homeostatis, muscle morphogenesis	3.26E-04	***
			TpnC73F	FBgn0010424	Calcium ion binding	3.01E-10	***
			Gpo-1	FBgn0022160	Glycerol-3-phosphate dehydrogenase activity, calcium ion binding	0.000209	***
INTERPRO, GOTERM_MF/BP/CC_DIRECT	Fatty acyl-CoA reductase, peroxisome	2.47	wat	FBgn0039620	Long-chain-fatty-acyl-CoA reductase activity, oxidation-reduction process	1.21E-06	***
			Spat	FBgn0014031	Amino transferase activity, glyoxylate catabolic process, proxisome	2.02E-02	*
			Cat	FBgn0000261	Catalase, antioxidant activity and ROS metabolic process	1.15E-02	*
			Uro	FBgn0003961	Urate oxidase activity, oxidation-reduction process, peroxisome	1.96E-02	*
			Acox57D-d	FBgn0034629	acyl-CoA dehydrogenase activity, fatty acid beta-oxidation, peroxisome	4.63E-05	***
GOTERM_MF/BP/CC_DIRECT, INTERPRO	Oxidation−reduction, haeme binding, iron ion binding, Cytochrome P450	2.2	Men	FBgn0002719	Malic oxidoreductase, determination of adult lifespan	5.16E-04	***
			Cyp6a23	FBgn0033978	Cytochrome P450, heme and iron ion binding, oxidation-reduction process	4.93E-01	NS
			Cyp4p1	FBgn0015037	Cytochrome P450, heme and iron ion binding, oxidation-reduction process	7.98E-02	.
			Cpr	FBgn0015623	NADPH-hemoprotein reductase activity, oxidation-reduction process	2.27E-04	***
			Cyt-b5-r	FBgn0000406	Heme binding, oxidoreductase activity, lipid metabolic process	2.69E-04	***
			antdh	FBgn0026268	Oxidoreductase activity, oxidation-reduction process	4.85E-02	*
			AOX1	FBgn0267408	Aldehyde oxidase, pyridoxal oxidase activity	5.82E-03	**
			Cyt-b5	FBgn0264294	Cytocrome b5-like heme-binding site	6.47E-04	***
			Cyp313a1	FBgn0038236	Cytochrome P450, heme and iron ion binding, oxidation-reduction process	1.45E-02	*
			mt: CoI	FBgn0013674	Mitochondrial Cytochrome C oxidase, heme and iron binding	7.39E-02	.
			Aldh	FBgn0012036	Aldehyde dehydrogenase, oxidation-reduction	9.39E-03	**
			Cyp4e3	FBgn0015035	Cytochrome P450, heme and iron ion binding, oxidation-reduction process	1.27E-05	***
			hgo	FBgn0040211	Homogentisate 1, 2-dioxygenase activity, oxidation-reduction process	4.08E-03	**
			Pdh	FBgn0011693	Retinol and alcohol dehydrogenase activity, oxidation-reduction process	4.75E-03	**
			Desat1	FBgn0086687	Fatty acid desaturase, oxidation-reduction process	2.55E-05	***
			Plod	FBgn0036147	Iron ion binding, oxidoreductase activity, oxidation-reduction process	6.75E-04	***
			Fad2	FBgn0029172	Fatty acid desaturase, oxidation-reduction process, lipid metabolic process	1.15E-03	**
			Gpdh	FBgn0001128	Carbohydrate metabolic process, oxidation-reduction process	6.13E-04	***
GOTERM_BP/CC/MF_DIRECT	Calcium ion channel, transmembrane transport	2.13	trpl	FBgn0005614	Calcium channel activity, calcium ion transport	2.13E-02	*
GOTERM_BP/CC/MF_DIRECT	Calcium ion channel, transmembrane transport	2.13	trp	FBgn0003861	Plasma membrane cation channel, calcium channel	3.06E-09	***
GOTERM_BP/CC/MF_DIRECT	Voltage-gated potassium channel activity	2.03	Irk3	FBgn0032706	Potassium channel activity, potassium ion transport	3.88E-02	*
KEGG_PATHWAY, INTERPRO, GOTERM_BP/CC/MF_DIRECT	Fatty acid metabolism/biosynthetic process	1.93	ACC	FBgn0033246	Acetyl-CoA carboxylase activity, fatty acid biosybthetic process	3.44E-03	**
			Cyt-b5-r	FBgn0000406	Cytochrome b5-like heme binding, lipid metabolic process	6.47E-04	***
			Desat1	FBgn0086687	Fatty acid desaturase, oxidation-reduction process	2.55E-05	***
			Fad2	FBgn0029172	Fatty acid desaturase, oxidation-reduction process, lipid metabolic process	1.15E-03	**
			Acsl	FBgn0263120	Long-chain fatty acid-CoA ligase activity	1.03E-04	***
INTERPRO, GOTERM_MF_DIRECT	Carbohydrate binding	1.91	Clect27	FBgn0031629	Carbohydrate binding	4.40E-11	***
INTERPRO, GOTERM_MF_DIRECT	Carbohydrate binding	1.91	lectin-37Db	FBgn0053533	Galactose binding	2.68E-04	***
INTERPRO, GOTERM_MF_DIRECT	Potassium ion transport, oxalate transmembrane transporter activity	1.65	Irk3	FBgn0032706	Potassium channel activity, potassium ion transport	3.88E-02	*
			Irk2	FBgn0039081	Potassium channel activity, potassium ion transport	1.96E-08	***
			Dic1	FBgn0027610	Inorganic phosphate transmembrane transporter activity	3.33E-05	***
GOTERM_MF/BP/CC_DIRECT, INTERPRO	Neurotransmitter-gated ion-channel ligand binding	1.45	Cals	FBgn0039928	Calcium ion binding	2.29E-02	*
GOTERM_MF/BP/CC_DIRECT, INTERPRO, COG_ONTOLOGY	Receptor activity, carboxylesterase, lipid metabolism	1.35	alpha-Est7	FBgn0015575	Carboxylic ester hydrolase activity, lipid storage, determination of lifespan	2.49E-04	***
			ACC	FBgn0033246	Acetyl-CoA carboxylase activity, fatty acid biosybthetic process	3.44E-03	**
			Gs2	FBgn0001145	Glutamate catabolic process, neurotransmitter receptor metabolic process	1.46E-03	**
			Est-6	FBgn0000592	Carboxylic ester hydrolase activity	2.93E-02	*
			AcCoAS	FBgn0012034	Acetyl-CoA ligase activity	3.35E-04	***
GOTERM_MF/BP/CC_DIRECT, INTERPRO	Neurotransmitter transporter	1.32	NAAT1	FBgn0029762	Neurotransmitter transporter activity	2.06E-08	***
GOTERM_MF/BP/CC_DIRECT, INTERPRO	Neurotransmitter transporter	1.32	Eaat1	FBgn0026439	Glutamate: sodium symporter activity, determination of adult lifespan	2.06E-02	*

Genes that were significantly down-regulated in these data in HPLC compared to LPHC were compared to a background list of genes that were expressed and detected in these data. Analyses of variance were carried out on expression between all samples in this study (F0−F3 matched and mismatched) with P-values and statistical significances listed.