Table 2.

Relative transcript abundance of selected homologs of Cry receptor proteins in the midgut of BPH

Deduced gene	RPKM	Relative abundance (△CT±SD)
bphcadherin1	5.59	6.68 ± 0.86
bphcadherin2	20.39	7.41 ± 0.94
bphcadherin3	3.7	5.45 ± 0.75
bphcadherin4	0.69	2.08 ± 0.89
bphAPN1	1182.63	10.88 ± 0.23
bphAPN2	5.05	3.19 ± 0.37
bphAPN3	2.00	3.18 ± 0.45
bphAPN4	1263.03	10.25 ± 0.24
bphAPN5	10.39	5.39 ± 0.50
bphALP1	0.29	1.08 ± 0.34
bphALP2	9.59	1.45 ± 0.41
bphALP3	0.59	0.76 ± 0.52
bphALP4	302.37	8.67 ± 0.29
bphALP5	2.06	1.70 ± 0.41
bphALP6	2020.66	11.67 ± 0.22

Reads Per Kilo base per million mapped reads (RPKM) of was calculated to predict transcript abundance of putative Bt receptor genes. The relative abundance (△Ct) of selected APNs, ALPs, and cadherins in the midgut was normalized using the BPH 18s rRNA threshold cycle (Ct) values that were obtained from reactions run on the same plate.