Fig. 6.
Western blotting was used to quantify the effect of a high-fat diet on IP3 and BKCa channel expression in aortic homogenates. a, b For IP3 expression, 3 different protein loads were added in adjacent lanes for each sample, with GAPDH used as the housekeeping gene. Two representative blots are shown. b Histogram showing a significant reduction in IP3 receptor expression after 4 months of a high-fat diet. n = 3. *** p < 0.001 vs. the C57BL/6 group. c, d For BKCa expression, α-actin was used as the housekeeping gene. d Histogram showing a significant increase in BKCa expression in aortic homogenates from ApoE−/− on the diet for 2–3 months, with no significant change at 4 months. Representative blots are shown. n = 3. ** p < 0.01 vs. the C57BL/6 group. 2m, 2 months of high-fat feeding; 3m, 3 months of high-fat feeding; 4 m, 4 months of high-fat feeding.