Table 2.

Gene Regulation Differences between Social Amoebae, Protostelium aurantium, and Other Amoebozoa

Gene Set	Dictyostelium sp.	Protostelium aurantium			Developmental Expression in P. aurantium			Physarum polycephalum	Acanthamoeba castellanii
	Development	Genes with Similarity (score >200)	% of Dictyostelium sp. Genes	Multiple Hits	Up	Down	Neutral	Genes with Similarity (score >200)	Genes with Similarity (score >200)
Upregulated during development	783	377	48	96	128	199	50	370	310
Essential for development	309	234	76	62	55	138	41	233	193
Upregulated and essential	75	57	78	13	21	21	15	57	47
All	1,167	668	57	171	204	358	106	660	550

Note.—About 1,167 genes of D. discoideum were identified previously as being either developmentally upregulated (Schilde et al. 2016), developmentally essential (Glöckner et al. 2016), or both (commonly identified in both studies). These genes were searched in the genomes of P. aurantium, Ph. polycephalum, and A. castellanii. To reduce spurious hits a BLAST similarity bit score of 200 was applied when comparing the encoded amino acid sequences. The column “Multiple hits” counts the occasions where different D. discoideum genes yielded the same P. aurantium hit.