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Duplex polymerase chain reaction (PCR) for detection of Mycoplasma pneumoniae and Legionella pneumophila: (A) Standardization of Duplex PCR targeting M. pneumoniae P1 adhesin gene 543 bp and L. pneumophila mip gene 375 bp. (B) Analysis of DNA from patient samples for P1 gene of M. pneumoniae and mip gene of L. pneumophila using duplex PCR.
