Table 1.
Sample set | Analysis | ||||
---|---|---|---|---|---|
Sensitivity testing | Specificity testing | Platform comparison | Precoated vs. freshly coated plates—ELISA | ROC analysis | |
Nepal samples of all ages | 0 | 0 | 424 | 424 | 0 |
Tanzanian samples of all ages for ROC—previously classified as Pgp3 antibody-positive or negative by MBA | 0 | 0 | 0 | 66 | 66 |
Tanzanian samples from PCR-positive children | 103 | 0 | 0 | 103 | 0 |
MIF (+) nonendemic children | 0 | 0 | 1 | 1 | 0 |
MIF (−) nonendemic children | 0 | 154 | 154 | 154 | 0 |
Total | 103 | 154 | 579 | 748 | 66 |
ELISA = enzyme-linked immunofluorescence assay; MBA = multiplex bead array; MIF = microimmunofluorescence assay; PCR = polymerase chain reaction; Pgp3 = protein plasmid gene product 3; ROC = receiver operator characteristics. Each sample set is also described in the Methods Section. Note that there were some analyses that used a combination of sample sets.