Table 4.

Pgp3 ELISA classification of samples using two different cutoff methodologies

Pre-coated ELISA plates
	Pos MM	Neg MM
Pos ROC	331	0
Neg ROC	52	365

Freshly coated ELISA plates
	Pos MM	Neg MM
Pos ROC	343	0
Neg ROC	56	349

ELISA = enzyme-linked immunosorbent assay; Pgp3 = protein plasmid gene product 3; MM = cutoff estimated using mixture model; Neg = negative classification (equal to or below the estimated cutoff threshold); Pos = positive classification (above the estimated cutoff threshold); ROC = cutoff estimated using receiver operator characteristic curve. Numbers in each box represent N per classification out of 748 samples total tested by ELISA. Top panel shows data from ELISAs using precoated plates, bottom panel shows data from ELISAs using same samples on freshly coated plates.