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. Author manuscript; available in PMC: 2018 Feb 8.
Published in final edited form as: Nature. 2017 Apr 26;545(7652):54–59. doi: 10.1038/nature22330

Figure 4. Functional integration of interneurons in fused hSS-hCS.

Figure 4

(a) Isolation of Dlxi1/2b::eGFP+ cells for transcriptional analysis. (b) t-SNE visualization of single cell gene expression at day 121 (4 weeks after hSS-hCS assembly). (c) Distribution across clusters (χ2-test, χ2= 43.39, P< 0.0001). (d) Expression of ERBB4, NXPH1, IGF1, TCF4, FOS, RAD1. (e) Morphology of Dlxi1/2b::eGFP+ cells before and after migration into hCS (f) Action potential generation in Dlxi1/2b::eGFP+ cells (one-way ANOVA, F(2, 30)= 1.25; ***P< 0.001; Bonferroni post-hoc, **P< 0.01; ***P< 0.001). (g) GABAergic synapse (by AT) on the pallial side of hCS-hSS with SYN1, GPHN and VGAT. (h) EPSCs and IPSCs in Dlxi1/2b::eGFP+ cells after migration. (i) Synaptic responses in Dlxi1/2b::eGFP+ cells (two-way ANOVA, interaction F(2, 61)= 18.46, P< 0.0001; Bonferroni post-hoc for EPSCs, ***P< 0.0001, **P< 0.001). (j) Synaptic responses in excitatory cells (two-way ANOVA, cortical neurons in hCS before and after assembly F(1, 26)= 5.6, P< 0.05; Bonferroni post-hoc for IPSC, *P< 0.05).