Extended Data Figure 2. Expression of Semaphorin receptors in geniculate ganglion neurons.

a, Quantitative RT-PCR analysis showing expression of candidate Sema3A receptors^5,6 (Nrp1, PlxnA3, and PlxnA4) and Sema7A receptors^5,6 (Itgb1 and PlxnC1) in the geniculate ganglia. P2x3 and T1r3 were used as controls for geniculate ganglia³⁵ and TRC⁹ tissue integrity, respectively. Nrp1, Neuropilin 1; PlxnA3, Plexin A3; PlxnA4, Plexin A4; Itgb1, Integrin β1; PlxnC1, Plexin C1. Data are presented for each gene as its relative abundance in the ganglia compared with TRCs. Values are mean + s.e.m. (n = 3). b-d, Confocal microscopy images of immunostains illustrating the expression of Nrp1 (c) and PlxnC1 (d) in subsets of geniculate ganglion neurons. Panel b depicts the anatomy of the ganglion highlighting the location of fiber tracks.