Table 3. Kinetic parameters of anti-FGFR2 scFv fragments.
Antibody name | KD (M)a | kon (1/Ms)a | koff (1/s)a |
---|---|---|---|
scFvD8 | 1.4 2x 10−7 | 5.39 x 104 | 7.65 x 10−3 |
scFvF7 | 3.70 x 10−8 | 1.06 x 105 | 3.99 x 10−3 |
scFvG3 | 5.93 x 10−8 | 9.86 x 104 | 5.85 x 10−3 |
scFvG9 | 7.60 x 10−8 | 7.12 x 104 | 5.43 x 10−3 |
scFvH6 | 3.85 x 10−7 | 2.53 x 104 | 9.75 x 10−3 |
a Measured on the BIAcore3000 instrument. Kinetic constants were calculated with the BIA evaluation 4.1 software. Fitting error for global KD fit was below 2,5%.