Table 4. Kinetic constants of bivalent formats of scFvF7.
KD (M)a | kon (1/Ms)a | koff (1/s)a | |
---|---|---|---|
scFvF7 | 3.70 x 10−8 | 1.06 x 105 | 3.99 x 10−3 |
scFvF7 diabody | 1.82 x 10−9 | 1.34 x 106 | 2.50 x 10−3 |
scFvF7-Fc | 0.76 x 10−9 | 1.61 x 106 | 1.22 x 10−3 |
a Measured on the BIAcore3000 instrument. Kinetic constants were calculated with the BIA evaluation 4.1 software. Fitting error for global KD fit was below 2,5%.