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. 2018 Feb 8;13(2):e0192694. doi: 10.1371/journal.pone.0192694

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© 2018 Ahn et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — S. mutans (1 × 10⁸ CFU/ml) was grown on 96-well polystyrene plates at 37°C for 24 h in the presence or absence of (A) L. plantarum culture supernatant (Lp.sup) at the indicated concentrations; (B) 20% of Lp.sup, proteinase K-treated Lp.sup (ProK-Lp.sup), or heat-treated Lp.sup (Heat-Lp.sup); (C) 20% of Lp.sup or octyl-sepharose beads-treated Lp.sup (Octyl beads-Lp.sup); (D) 30 μg/ml of L. plantarum LTA (Lp.LTA), L. plantarum lipoprotein (Lp.LPP), L. plantarum peptidoglycan (Lp.PGN), MDP, or Tri-DAP. Biofilm formation extent was determined by a crystal violet assay. Data are mean values ± S.D. of triplicate samples. Asterisk, significant induction at P < 0.05 compared with non-treatment control group.