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. 2018 Feb 8;13(2):e0192722. doi: 10.1371/journal.pone.0192722

Fig 1. False-positive detection of bisulfite-resistant cytosines (brCs) in the negative control amplicons (NCAs) of mtDNA by bisulfite pyrosequencing.

Fig 1

BrCs were detected in gel-purified or unpurified, bisulfite-converted PCR amplicons by pyrosequencing for 15 CpG sites: 5 in human ND1, 5 in human CYTB, 2 in mouse ND1, and 3 in mouse CYTB. The p-value was calculated using paired t-test (two-tails). The lines show mean ± 95% Confidence Intervals.