Fig 7. Modulation of collagen 1a1 expression after induction/inhibition of SPARC in the mature myotubes.

C2C12 myoblasts were cultured in DM for 5 days. On day 6, exogenous rSPARC (4 μg/ml) and anti-SPARC (40 μg/ml) were added and kept for 48 h. Proteins extraction was performed on day 8. Total cell extracts (30 μg proteins per lane) were subjected to western blot analysis with anti-collagen 1a1 antibody. The experiments were repeated three times (three different passages of C2C12 cells) and a representative western blot image was shown. Data were expressed as a ratio to the positive control (pooled samples). One-way ANOVA with repeated measurements was used to adjust the validations of the different passages and a contrast analysis was performed. P value was set at < 0.05 after the Bonfferoni adjustments. Abbreviations: PBS: phosphate buffered saline, rSPARC: recombinant SPARC protein, and anti-SPARC: anti-SPARC antibody. *Significant differences between experimental conditions.