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. 2018 Jan 29;12(1):e0006203. doi: 10.1371/journal.pntd.0006203

Fig 4. Positions of the 10 clusters containing Lao strains on phylogenetic tree built with DENV-1 envelop sequences.

Fig 4

3,108 DENV-1 envelope sequences, obtained from the European Molecular Biology Laboratory (EMBL) Nucleotide Sequence Database in April 2014, were aligned with the DENV-1 sequences obtained in this study. A tree was built with this dataset using a maximum-likelihood (ML) method (General Time Reversible Model with invariant sites and a gamma distribution of rates across sites) and bootstrap resampling with 500 replicates. 81 Lao strains are dispersed in 10 clusters (bootstrap >70) shaded here in separate colors. The bootstrap values are displayed on the tree only for those clusters. The list of the strains grouped in the different clusters is provided in S6 Table. Two strains from this study do not belong to any of those clusters and are displayed in the tree. The Lao strain from 1996 is not shown on the figure and belongs to Asia 2 clade.