3,108 DENV-1 envelope sequences, obtained from the European Molecular Biology Laboratory (EMBL) Nucleotide Sequence Database in April 2014, were aligned with the DENV-1 Lao sequences obtained in this study. A tree was built (Fig 4) and ten clusters (bootstrap >70) containing the strains from this study were identified. In order to visualize the circulation of all the strains from these different clusters, we plotted each strain according to time of collection and location. Each of the 10 clusters is displayed in a different color: yellow for the strains from cluster # 1, light green for cluster #2, red for cluster #3, blue for cluster #4, dark green for cluster #5, medium green for cluster # 6, pink for cluster #7, grey for cluster #8, light orange for cluster #9 and dark orange for cluster #10. The list of all the strains included in each cluster is provided as S6 Table.