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. 2018 Jan 29;14(1):e1006880. doi: 10.1371/journal.ppat.1006880

Fig 4. Phenotypic analysis of Xe strains overexpressing XopAU.

Fig 4

Leaves of the pepper line ECW30R were syringe-infiltrated with a 10 mM MgCl2 mock solution or with suspensions (1 x 107 CFU/ml) of Xe strains carrying a vector either empty (EV) or for expression of XopAU-HA or XopAUK240A-HA. (A) Photograph of an inoculated leaf at 5 days post-inoculation (dpi). (B) Chlorophyll content relative to mock-inoculated areas at 3 and 5 dpi. (C) Electrolyte leakage in the inoculated areas at 3 and 5 dpi. In B and C, box plots display 25th, 50th (middle line) and 75th percentiles (n = 10). Asterisks indicate a significant difference (Mann-Whitney U test, p value <0.05) relative to Xe containing an empty vector. Experiments were repeated at least three times with similar results.