Fig 8. Expression of MKK2_K99R suppresses cell death mediated by XopAU.

N. benthamiana leaves were inoculated with Agrobacterium to co-express His-XopAU or an empty vector (EV) with MKK2-HA, MKK2_K99R-HA or GFP. Expression was driven by an estradiol-inducible system and 17β-estradiol was applied 24 h after agro-infiltration. (A) Photograph taken at 48 h after 17β-estradiol application. (B) Electrolyte leakage at 24 h and 48 h after 17β-estradiol application. A box plot displays 25^th, 50^th (middle line) and 75^th percentiles. (n = 5). Asterisks indicate a significant difference (Mann-Whitney U test, p value <0.05) relative to co-expression of His-XopAU with a GFP control. The experiment was repeated three times with similar results.