Fig 9. Co-expression of XopAU and MKK2 inhibits yeast growth.

Yeast cultures containing the pGML10 vector either empty (EV1) or for expression of XopAU and XopAU_K240A, and the pGMU10 vector either empty (EV2) or for expression of MKK2 and MKK2_K99R were normalized to OD₆₀₀ = 0.1, and serial dilutions were spotted onto selective media containing 2% glucose or 2% galactose and 1% raffinose. Plates were incubated at 30°C for 72 h (2% glucose) or 96 h (2% galactose and 1% raffinose) and photographed. The experiment was repeated three times with similar results.