Fig 1. Putative transcriptional components of the auxin-mediated PIN polarization.
(A) Simultaneous immunolocalization of PIN1 and PIN2 in HS::axr3-1 plants. Heat shock-induced overexpression of axr3-1 abolishes lateral PIN relocation after auxin (4 h, 10 μM NAA) treatment, confirming dependence on the SCFTIR1-Aux/IAA-ARF signalling pathway. Arrowheads highlight representative examples of PIN localization in the respective tissues and treatments (PIN1 in endodermis and PIN2 in cortex). Bar = 10 μm. epi, epidermis; co, cortex; en, endodermis. (B) Quantitative evaluation of (A), confirming reduced auxin-dependent relocation of PIN1 (top) and PIN2 (bottom) in the induced HS::axr3-1 line. Graph shows mean ratio of lateral-to-basal signal intensity of PIN1 in endodermal and PIN2 in cortex cells. Error bars indicate standard error. A One-Way ANOVA test compared marked sets of data. (** p<0.01; *** p<0.0001; n>35 cells corresponding to a minimum of 10 roots per treatment and experiment were imaged under comparable conditions). Experiments were carried out at least 3 times; one representative experiment is presented. (C) Scheme of the microarray experiment and analysis strategy.