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. 2018 Jan 29;14(1):e1007177. doi: 10.1371/journal.pgen.1007177

Fig 3. WRKY23 is required for auxin-mediated PIN lateralization in the root.

Fig 3

(A) Immunolocalization analysis of PIN2 without or after NAA (4 h, 10 μM) treatment in WT Col-0 and RPS5A>>WRKY23. Arrowheads highlight PIN2 polarity. epi, epidermis; co, cortex. (B) Quantitative evaluation of (A) showing mean ratio of PIN2 lateral-to-basal signal intensity in cortex cells. Note that PIN2 lateralization in RPS5A>>WRKY23 roots is increased even without auxin that still remains effective. Error bars indicate standard error. A One-Way ANOVA test compared marked sets of data (*** p<0.0001; n>35 cells corresponding to a minimum of 10 roots per treatment and experiment were imaged under comparable conditions). (C) Immunolocalization analysis of PIN2 without or with NAA treatment in WT Col-0 and wrky23 mutants. Arrowheads highlight representative examples of PIN2 polarity in the. epi, epidermis; co, cortex. (D) Quantitative evaluation of the experiment in (C) showing mean ratio of PIN2 lateral-to-basal signal intensity in endodermal. Error bars indicate standard error. A One-Way ANOVA test compared marked sets of data (* p<0.05; *** p<0.001; n>100 cells corresponding to a minimum of 10 roots per treatment and experiment were imaged under comparable conditions). Experiments were carried out 3 times; one representative experiment is presented).