A: Schematic synaptic connectivity. Edge weights were collected from www.wormwiring.org [8]. Only connections that directly involve PHC are shown.
B: PHC is required in hermaphrodites for response to harsh (picking-force) touch to the tail. Bar graph indicates the mean percentage of animals responding to harsh touch, with error bars indicating the minimum and maximum from two experimental replicates. Significance was calculated using Fisher’s exact test. ** p<0.01, n.s. p>0.05. “n” in each column indicates the total number of animals assayed.
C: PHC is required for a specific step of the male mating behavior, vulva search behavior. Mutant and Histamine-silenced animals tested for the male’s vulva location efficiency. Box plot representation of the data is shown, with whiskers pointing from min to max. Median and mean values are indicated by horizontal line and “+”, respectively. Statistics was calculated using Kruskal-Wallis test. ****P < 0.0001, ***P < 0.0005. “n” above graphs indicates the total number of animals assayed.