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. Author manuscript; available in PMC: 2018 Feb 8.
Published in final edited form as: Nat Genet. 2017 Sep 25;49(11):1602–1612. doi: 10.1038/ng.3963

Figure 1.

Figure 1

HiChIP identifies high-resolution chromosome conformation in primary human T cells. (a) Primary T cell H3K27ac HiChIP experimental outline. (b) Left, FACS strategy for naive, TH17, and Treg cells from total peripheral blood CD4+ T cells. Number represents the percentage of total CD4+ T cells within that gate. Right, Knight–Ruiz (KR) matrix–balanced interaction maps for naive, TH17, and Treg cells at 500-kb, 25-kb, and 5-kb resolution, and raw interaction maps at 1-kb resolution, centered on the KLF2, RBPJ, and LRRC32 loci. (c) HiChIP 1D and 3D signal enrichment at the RORC locus in TH17 over naive T cells.