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. 2018 Jan 4;6(2):2037–2046. doi: 10.1021/acssuschemeng.7b03451

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Copyright © 2018 American Chemical Society

This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.

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Normalized MS peak areas in time of reaction products formed upon incubation of GBG with laccase alone (A), laccase/HBT (B), and laccase/ABTS (C). Areas were obtained from extracted ion chromatograms: GBG (m/z 343) (black dot), GBG dimer (m/z 637) (red dot), GBG trimer (m/z 955) (blue dot), GBG-HBT CP (m/z 452) (grey dot), GBG-HBT CP_ox (m/z 450) (open dot, grey line), GBG_ox (m/z 319 and 341) (open dot, black line), GBG-ABTS CPI_ox (m/z 414) (green dot), GBG-ABTS CPII (m/z 588) (purple dot), GBG-ABTS CPII_ox (m/z 586) (open dot, purple line) and ABTS CLP (m/z 258) (orange dot). For the abundance (Y axis), the time point with the largest peak area was set to 100%. For the other time points, the abundance was calculated as the peak area relative to the area of this largest peak. Amounts withdrawn per time point and injected to UHPLC-MS analysis were the same for all time points.