Table 3.
SHR mean ± SEM |
WKY mean ± SEM |
Strain p-value |
E2 p-value |
Strain × E2 p-value |
|
---|---|---|---|---|---|
Genes | |||||
Chymase mRNA | 0.0002 | 0.0001 | 0.0011 | ||
Intact | 0.47 ± 0.06 | 0.58 ± 0.16 | |||
OVX | 0.72 ± 0.10 | 1.78 ± 0.13* | |||
ACE mRNA | 0.05 | 0.024 | 0.613 | ||
Intact | 0.82 ± 0.16 | 0.42 ± 0.05 | |||
OVX | 1.12 ± 0.19 | 0.88 ± 0.08 | |||
ACE2 mRNA | 0.51 | 0.57 | 0.48 | ||
Intact | 0.5 ± 0.08 | 0.49 ± 0.22 | |||
OVX | 0.47 ± 0.12 | 0.72 ± 0.32 | |||
Angiotensinogen mRNA | 0.104 | 0.012 | 0.896 | ||
Intact | 0.71 ± 0.17 | 0.42 ± 0.05 | |||
OVX | 1.12 ± 0.19 | 0.88 ± 0.08 | |||
AT1R mRNA | 0.031 | 0.331 | 0.273 | ||
Intact | 1.34 ± 0.10 | 1.56 ± 0.23 | |||
OVX | 1.32 ± 0.10 | 1.94 ± 0.29 | |||
Mas mRNA | 0.0001 | 0.0001 | 0.0007 | ||
Intact | 1.05 ± 0.08 | 1.73 ± 0.32 | |||
OVX | 1.28 ± 0.12 | 3.98 ± 0.46* | |||
ANF mRNA | 0.513 | 0.573 | 0.484 | ||
Intact | 0.50 ± 0.08 | 0.49 ± 0.22 | |||
OVX | 0.47 ± 0.12 | 0.72 ± 0.32 | |||
BNP mRNA | 0.22 | 0.015 | 0.21 | ||
Intact | 0.63 ± 0.15 | 0.32 ± 0.10 | |||
OVX | 0.79 ± 0.12 | 0.80 ± 0.06 | |||
NOX2 mRNA | 0.037 | 0.006 | 0.047 | ||
Intact | 0.91 ± 0.03 | 0.92 ± 0.08 | |||
OVX | 0.98 ± 0.07 | 1.30 ± 0.13† | |||
SERCA2 mRNA | 0.0001 | 0.973 | 0.63 | ||
Intact | 1.65 ± 0.11 | 0.85 ± 0.19 | |||
OVX | 1.59 ± 0.13 | 0.93 ± 0.09 | |||
PLB mRNA | 0.0001 | 0.006 | 0.638 | ||
Intact | 1.21 ± 0.14 | 0.36 ± 0.06 | |||
OVX | 1.65 ± 0.14 | 0.69 ± 0.08 | |||
PLB/SERCA2 mRNA | 0.74 ± 0.08 | 0.47 ± 0.05 | 0.0004 | 0.0002 | 0.881 |
Intact OVX | 1.40 ± 0.04‡ | 0.75 ± 0.06† | |||
Proteins | |||||
AT1R | 0.0001 | 0.0002 | 0.0814 | ||
Intact | 3.12 ± 0.28 | 1.84 ± 0.11 | |||
OVX | 1.95 ± 0.08§ | 1.34 ± 0.07 | |||
Mas | 0.0005 | 0.006 | 0.053 | ||
Intact | 2.43 ± 0.16 | 1.65 ± 0.05 | |||
OVX | 1.79 ± 0.12∥ | 1.52 ± 0.08 |
Cardiac gene transcripts and protein expression were measured in SHR and WKY hearts 10 weeks after OVX or sham procedures (gonads intact). The groups consisted of intact-SHR (n = 6), OVX-SHR (n = 5), intact-WKY (n = 5), OVX-WKY (n = 4). Using a two-way ANOVA, significant differences in cardiac gene and protein expression, with respect to strain, estrogen (E2) status (intact versus OVX), and strain x E2 status, were determined. The p-values are presented on the right of the table. For convenience, significant differences are indicated in bold. Post-hoc analysis using Tukey’s multiple comparisons indicate significant differences within-strain for estrogen status.
p < 0.0001 OVX-WKY versus intact-WKY;
p < 0.05 OVX-WKY versus intact-WKY;
p < 0.01 OVX-SHRs versus intact-SHR;
p < 0.001 OVX-SHRs versus intact-SHRs;
p < 0.01 OVX-SHRs versus intact-SHRs.
ACE: angiotensin converting enzyme; ACE2: angiotensin converting enzyme 2; AT1R: angiotensin receptor 1; ANF: atrial natriuretic factor; BNP: brain natriuretic peptide; NOX2: NADPH oxidase 2; OVX: ovariectomy; PLB: unphosphorylated phospholamban; RAS: renin–angiotensin system; SEM: standard error of the mean; SERCA2: sarcoplasmic-endoplasmic calcium ATPase 2; SHR: spontaneously hypertensive rats; WKY: Wistar-Kyoto rats.