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. 2017 Dec 30;9(6):6814–6829. doi: 10.18632/oncotarget.23784

Figure 1. TLR2 and A2a are expressed together in oral ED and SCC cells.

Figure 1

Examples of SCC, two from the tongue (A, B) and one from the gingiva (C). Serial (back-to-back) sections of archival paraffin-embedded human samples were processed and stained by IHC as described in Materials and Methods. The TLR2-positive and A2a-positive cells are stained brown and nuclei are blue. Note similar expression of TLR2 and A2a throughout the dysplastic epithelium and SCC. An internal control, nerve fibers, are A2a-positive and TLR2-negative (B, Lower row, arrows). For negative controls (top panels), primary TLR2- and A2a-specific antibodies were replaced with species-, isotype- and concentration-matched nonspecific antibodies.