Table 3. TLR2 and/or AR stimuli induce Ki-67 mRNA expression in OSCC cells by 24 hrs.

stimuli	KER	PCI13	CAL27	UMSCC19
none	1.00 ± 0.02	1.00 ± 0.03	1.00 ± 0.05	1.00 ± 0.02
TLR2/1+2/6	1.15 ± 0.03*	3.02 ± 0.63***	1.52 ± 0.04****	1.11 ± 0.02**
AR	1.08 ± 0.07	2.03 ± 0.35*	1.45 ± 0.003****	1.03 ± 0.04
TLR2/1+2/6+AR	0.94 ± 0.08	1.49 ± 0.27*	2.11 ± 0.05****	1.01 ± 0.04
EGF	1.52 ± 0.04****	3.14 ± 0.78***	1.42 ± 0.05****	0.70 ± 0.02**

Fold change relative to unstimulated cells, ± SD.

Bold = significant; ^*p < .05; ^**p < .01; ^***p < .001; ^****p < .0001.

OSCC cells Cal27, PCI13 and UMSCC19, and keratinocytes hTERT HAK Clone 41 were incubated for 2, 4, and 24 hrs with EGF, or with one or more of the TLR2/1, 2/6 ligands and AR agonist NECA, as indicated in Materials and Methods. Cellular mRNA was evaluated for Ki-67 and GAPDH expression by qRT-PCR, in triplicate. Fold changes relative to unstimulated cells ± SD are shown. Data were analyzed using one way ANOVA, including Tukey-Kramer test for multiple comparisons.