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. 2017 Dec 15;9(6):7023–7035. doi: 10.18632/oncotarget.23317

Figure 4. Metformin (Met) reduces cancer stem-like properties generated by induction of TGF-β1.

Figure 4

(A) Metformin inhibited gliosphere formation in LN18 and U87 cells stimulated by exposure to TGF-β1 (10 ng/ml). Representative images of gliosphere were photographed under Olympus microscope (×100 magnification). Histograms show the numbers of gliosphere in different treatment groups. (B) Western blot results of inhibitory effect of metformin on stemness-related proteins stimulated by exposure to TGF-β1 in LN18 and U87 cells. **P < 0.01, ***P < 0.001, TGF-β1 treated group vs. control group; TGF-β1 treated group vs. TGF-β1 and Metformin (10 mM) treated group.