Table 1. List of NanoString PAM50 MTE primers.
| Gene name | Forward sequence | Reverse sequence |
|---|---|---|
| ACTB* | Unable to design primers around probe region | |
| ACTR3B | ATGTGGATCCCCGGAAGT | TCGATGGGGCAGTTCTGT |
| ANLN | GAACCACCGTTTCCATCG | AGAGAGGGGCTGCTGGTT |
| BAG1 | AGGAGGTGACCAGGGAGG | TCCAAGTGCTGACAACGG |
| BCL2 | CTCCTGTCTTTGGAAATCCG | AACACCTTCTCCCCAGCC |
| BIRC5 | CAGAGTCCCTGGCTCCTCT | GGCTCACTGGGCCTGTC |
| BLVRA | AACTGTGGGAGCTGGCTG | TCCCCAAAGAGGGAGACC |
| CCNB1 | TGGATGCAGAAGATGGAGC | GGTCTCCTGCAACAACCTG |
| CCNE1 | TACTTGCTGCTTCGGCCT | TCCAAGCTGTCTCTGTGGG |
| CDC20 | CAGCAGCAGAAACGGCTT | GGCCAAATGTCGTCCATC |
| CDC6 | TGCTCTTGATCAGGCAGTTG | TGTCAAACGGGTTTCCTTG |
| CDH3 | CCAGACAGTGGGCAGGTC | GATGGTGATCTGACGGGG |
| CENPF | GCCTGGCTGCACAGAAGT | ACCCTCAGGCCCTCTCTG |
| CEP55 | CGAGTCCTTGAGGCTGAGA | TCGTGAGGTTGCAGCAGA |
| CXXC5 | GGTGGTTTCAGTGACGGC | TGCTAGGGACGTGGAGATG |
| EGFR | TACTTGGAGGACCGTCGC | TCACCCCGTAGCTCCAGA |
| ERBB2 | ACACCTAGCGGAGCGATG | TCTTTGTTGGCTTTGGGG |
| ESR1 | ATGGAGCACCCAGGGAA | CTTCAGGGTGCTGGACAGA |
| EXO1 | GGGGAAAGTCTCGGAAGC | GCCAAAAGCTAGGAGATCCG |
| FGFR4 | GGAGCTGCTGTGCAAGGT | GTCCTCGGCTGACACGTT |
| FOXA1 | AGCTGCTCAGCTCCCCTC | GGAGTTCATGGAGCCCAG |
| FOXC1 | GCCAGCAGCAGAACTTCC | AACTTGCTACAGTCGTAGACGAAA |
| GPR160 | CCAAGCTTTCATTTAAGTGTCAAA | CCATGAAAAATGACAGCCAG |
| GRB7 | CAGGACGGAAGCTTTGGA | TTTCGAAGCTTGTTGGGC |
| GUSB* | CCACATGCAGGTGATGGA | ATACGGAGCCCCCTTGTC |
| KIF2C | GGAGAACCAAGCATTCTGC | TGGATGCATTCTGGGCTT |
| KRT14 | GCAGGAGATCGCCACCTA | GGCAGCCTCAGTTCTTGG |
| KRT17 | TGCTGGAGGGAGAGGATG | CCGGGGTAGCTGAGTCCT |
| KRT5 | CTCTCCAGCACCTCCCAA | ACGGAGGTGAAGCTGGTG |
| MAPT | CCCCTCTTTGGGAGAGGA | TGTTTGGGGCTAAGGCAA |
| MDM2 | CGTGAAGGAAACTGGGGA | TTTTTGTGCACCAACAGACTTT |
| MELK | ACGGAGATTGAGGCCTTG | TCCCTGTGAGCATAGCCC |
| MIA | TGCCGATTCCTGACCATT | CGGACAATGCTACTGGGG |
| MKI67 | CCAGTGATCAACGCCGTA | CATCTGCCCATGATTTTGC |
| MLPH | GTGAAGCCCTCGGGAAAG | GCGAGCCTCGGTACACTG |
| MMP11 | TGACCAGGGCACAGACCT | CCTGGAGGTGACAGTGGG |
| MRPL19* | GGAGAAAAGTACTCCACATTCCA | CTCCTGGACCCGAGGATT |
| MYBL2 | TCTTGCTGGACCGAGGAG | GGGCTTGCAGTCTTTGGA |
| MYC | AACCGAAAATGCACCAGC | CTCCTCTGCTTGGACGGA |
| NAT | Unable to design primers around probe region | |
| NDC80 (KNTC2) | GTCCCTGGGTCGTGTCAG | TTTCTCTTTGGTTTGAGGGG |
| NUF2 (CDCA1) | CCTGCAGACAGACGCCTT | GGTTTTTACCATCAGCTCCTGT |
| ORC6 | AATGGTAGCCACATCCGGT | TTCTCCATTTCCTTTGCTGG |
| PGR | TCAGAAGCCAGCCAGAGC | CTAAGGCGACATGCTGGG |
| PHGDH | TGCCGCAGAACTCACTTG | CTGGGGAAATGATGGGGT |
| PSMC4* | CAGGAGGAGGTGAAGCGA | GCATTGCTGTGCTTGTGG |
| PTTG1 | CAACACCACGTTTTGGCA | GGCATCATCTGAGGCAGG |
| PUM1* | TCCTGGGTGATCAATGGC | CTTGGGCCAAATCCTCCT |
| RPLP0* | GCGACCTGGAAGTCCAAC | TGTTTTCCAGGTGCCCTC |
| RRM2 | CGCCGCTTTGTCATCTTC | GGCTAAATCGCTCCACCA |
| SF3A1* | CTTCTTGACCCTCGCTGG | TGGATCTCCTCCTCACCG |
| SFRP1 | CCCACCTTTCAGTCCGTG | ATGGCTACCCTGGGGTTT |
| SLC39A6 | GGAGATTAAGAAGCAGTTGTCCA | ATTCTTGCACCCTCTGGG |
| TFRC* | GGACATGCTCATCTGGGG | TCTGTTTTCCAGTCAGAGGGA |
| TMEM45B | CACCTTTTGGAACACCCG | CAAGAGGGCGGTCTGGTA |
| TYMS | GGAAGGGTGTTTTGGAGGA | CCCAAAATGCCTCCACTG |
| UBE2C | CCTGCTATCACCCCAACG | CTGGCTGGTGACCTGCTT |
| UBE2T | TGGTACCCCGTTGGTCC | GGGTGGCTCTGTGGCTAA |
* Reference genes.