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. Author manuscript; available in PMC: 2019 Feb 14.
Published in final edited form as: Neurosci Lett. 2017 Dec 12;666:1–4. doi: 10.1016/j.neulet.2017.12.027

Fig. 2.

Fig. 2

Knockdown of Cx36 prevents neuronal death caused by overexpression of the SOD1-G93A mutant protein. Statistical data from MTT assay (A) and representative images from western blot experiments (B, C) in WT mouse purified neuronal spinal cord cultures are shown. The cultures were transduced with shRNA-LUC or shRNA-Cx36 and also were transfected with the empty vector (VECT) or plasmids inducing human SOD1 or G93A mutant. shRNA-Cx36 effectively suppressed Cx36 protein expression (B). Cultures transfected with the SOD1 and G93A mutant demonstrated the FLAG reactivity (C). Statistical analysis in A: ANOVA with post hoc Tukey; n = 8 per group in all groups; *P<0.05; NS, non-significant.