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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Mol Cancer Ther. 2017 Nov 22;17(2):443–454. doi: 10.1158/1535-7163.MCT-17-0560

Figure 5. CA3 suppresses ALDH1+ cell tumor sphere and exerts strong antitumor effects in inducible high YAP xenograft model.

Figure 5

A. ALDH1 positive or negative cells were sorted from JHESO EC cells and tumor sphere assays were done in the sorted cells and add CA3 at 0.5µM at the beginning of the tumor sphere culture. After 8–10 days of culture, the tumor sphere numbers formed were counted under microscope. Representative fields (top) and the bar graph (low) were demonstrated. B–D. SKGT-4 (PIN20YAP1) cells with (DOX+) or without (DOX−) YAP1 induction were inoculated into nude mice of both sites (n = 5 per group). Representative tumors after 6 weeks are shown (B).Tumor weight (C) and body weight(D) were calculated as described in Materials and Methods after 6 weeks. E. immunohistochemistry for YAP1, SOX9, and Ki67 was performed in mouse tumor tissues derived from xenograft nude mice. Scale bar, 50 µm; 200× magnification.