Fig. 1.

Scheme of multicenter experiment. A. Outline of reagent preparation for each site. A master cocktail was mixed from 20 undiluted antibodies. Six aliquots were made for distribution, then the remaining cocktail was used to stain PBMCs to make Prestained sample aliquots for each site. An unstained PBMC aliquot from the same donor was also shipped for staining on-site. B. Protocol for staining cells and sample acquisition. One PBMC aliquot was thawed and split, then stained on Day 1. On Day 2, each sample was split into two parts. Part 1 was run on the CyTOF that day, while part 2 was run on Day 3. Additionally, aliquots of Prestained sample were thawed and run each day.