Table 5.
Retention of long peptides with and without a column oven
| Peptide | Length | Predicted RT (GU) | RT (Without Oven) | RT (With 60° Oven) |
|---|---|---|---|---|
| RPCFSALTPDETYVPK | 16 | 6.39 | 8.28 | 6.63 |
| LFTFHADICTLPDTEK | 16 | 5.05 | 7.40 | 4.56 |
| NTDGSTDYGILQINSR | 16 | 6.27 | 6.43 | 6.35 |
| EDLIWELLNQAQEHFGK | 17 | 5.47 | 5.39 | 5.05 |
| GITWGEETLMEYLENPK | 17 | 4.33 | 3.35 | 4.04 |
| VYACEVTHQGLSSPVTK | 17 | 6.69 | 2.37 | 4.07 |
| TTPPVLDSDGSFFLYSK | 17 | 3.19 | 4.14 | 3.18 |
| GITWGEETLMEYLENPKK | 18 | 6.45 | 4.66 | 6.52 |
| TVAAPSVFIFPPSDEQLK | 18 | 3.65 | 4.22 | 3.92 |
| RTVAAPSVFIFPPSDEQLK | 19 | 5.49 | 5.78 | 4.92 |
| AAPSVTLFPPSSEELQANK | 19 | 5.77 | 5.81 | 5.18 |
| ANPTVTLFPPSSEELQANK | 19 | 6.24 | 6.00 | 5.87 |
| EVQLVQSGGGLVQPGGSLR | 19 | 4.84 | 6.11 | 4.60 |
| DLILQGDATTGTDGNLELTR | 20 | 6.27 | 7.38 | 5.73 |
| VDNALQSGNSQESVTEQDSK | 20 | 10.70 | 10.54 | 10.04 |
| GLVLIAFSQYLQQCPFDEHVK | 21 | 4.01 | 5.65 | 4.69 |
| GFYPSDIAVEWESNGQPENNYK | 22 | 6.82 | 7.08 | 6.13 |
| SPDSHPADGIAFFISNIDSSIPSGSTGR | 28 | 7.65 | 8.54 | 5.62 |