Figure 2. ETV4 enhances pancreatic cancer cell growth and clonogenic ability.

(A) Stable ETV4-silenced (Colo357-shETV4 and ASPC1-shETV4) and overexpressed (BXPC3-ETV4) cell lines with their scrambled control (Colo357-NTScr and ASPC1-NTScr and BXPC3-Neo) cells were generated, and ETV4 expression was examined by immunoblot analyses. (B) For growth kinetics, cells (1×10⁴) were seeded in 6-well plates and growth was monitored by counting the cell number up to 8 days. (C) For anchorage-dependent colony formation assay, cells were seeded at low density (1×10³cells/well) in regular media. After 2 weeks, colonies were stained with crystal violet and visualized and photographed using imaging system. Bars represent mean ± S.D. n=3; *, p< 0.05.