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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Mol Cancer Res. 2017 Nov 8;16(2):187–196. doi: 10.1158/1541-7786.MCR-17-0219

Figure 3. ETV4-silencing arrests cell cycle in G1/S phase by regulating the expression of genes involved in regulation and progression of cell cycle.

Figure 3

(A) ETV4-silenced and -overexpressing cells were synchronized by culturing them in serum-free media for 48h and then incubated in regular medium for 24h. Subsequently, cells were fixed in methanol and stained with Propidium iodide. The distribution of cells in different phases of cell cycle was analyzed by flow cytometry. (B) Expression profile of selected cell cycle associated genes by quantitative RT-PCR upon ETV4 silencing in Colo357 cells and (C) expression of Cyclin D1 at mRNA and protein level in low and high ETV4-expressing Colo357, ASPC1 and BXPC3 cells. GAPDH and β-actin were used as loading controls.