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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Mol Cancer Res. 2017 Nov 13;16(2):296–308. doi: 10.1158/1541-7786.MCR-17-0308

Figure 6. CCL2 derived from DCIS fibroblasts is important for progression of DCIS.com breast cancer cells.

Figure 6

A. CCL2 ELISA of 1213-249 fibroblasts expressing control shRNA (Con) or CCL2 shRNAs from pLenti or GIPZ lentivirus systems. B-C. Control or CCL2 deficient 1213-249 fibroblasts were co-grafted with DCIS.com breast cancer cells in the subrenal capsule and analyzed for changes in tumor growth (B), and scored for tumor invasion into normal kidney tissue by pan-cytokeratin (red) and phalloidin (green) staining (C). N=7 mice/group. Scale bar=400 microns. K=kidney, T=tumor. Statistical analysis was performed using One way ANOVAwith Bonferonni post-hoc comparison. Statistical significance was determined by p<0.05. **p<0.01. Mean±SEM are shown.