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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Hum Immunol. 2017 Dec 14;79(2):77–86. doi: 10.1016/j.humimm.2017.12.004

Table 2.

Validation and Database Functions Applied to each typing report format

HML Validation

  1. GL-String content validation with strict-mode GL service of the provided Reference Database version

  2. GL-String “sanity check” syntax validation
Functions

  1. GL-String LiftOver to Reference Database version 3.25.0

  2. GL-String concatenation

  3. Retrieve HLA typing from GL-String using the provided reference allele
GenDx Functions

  1. Generate GL-String from GenDx “genotype list” elements

  2. Generate phasing groups
IHIW XML/Manual Entry Validation

  1. Identifier (e.g. 17th IHIW labcode, sample ID) validation

  2. GL-String validation with strict-mode 3.25.0 GL service

  3. The uniqueness of the quartet annotation (sample ID, HLA typing, phasing group, start position) of consensus sequences

  4. IUPAC nucleotide code[50] validation for consensus sequence
Functions

  1. hlaPoly application to identify novel polymorphisms

HML: Histoimmunogenetics Markup Language

IHIW: International HLA and Immunogenetics Workshop

IUPAC: International Union of Pure and Applied Chemistry

XML: eXtensible Markup Language

Because HML and GenDx XML typing reports are converted to IHIW XML reports upon submission, the validation and functions listed for IHIW WS format are applied to all typing reports.

Validation results and details are provided in the WS Database system under “Lab Member”/”Tools”/”Job Log”.