(a) D. melanogaster tkv is expressed in nurse cells, stretch cells, and two dorsolateral patches of columnar follicle cells. (b) S. lebanonensis tkv is expressed in nurse cells, stretch cells, and 4–5 rows of dorso-anterior columnar follicle cells. (c) Quantitation of S. lebanonensis tkv expression; N > 10 for each stage category. (d) At S10A in D. melanogaster, P-Mad is present in a 2–3-row ring of follicle cells at the anterior of the oocyte. (e) At a slightly later stage in S. lebanonensis, P-Mad is present in a 4–5-row-wide ring of anterior follicle cells. Some cells have begun to migrate centripetally and are no longer visible in this plane. (f) This dorsolateral view of a S11 D. melanogaster egg chamber shows that P-Mad is restricted to two symmetrical patches (left patch marked by a solid curved line) on each side of the dorsal midline (dashed line). (g) By late S11/early S12 in S. lebanonensis, P-Mad expands posteriorly to 7 rows of follicle cells. (h) Quantitation of S. lebanonensis P-Mad localization; N > 10 for each stage category.