(a) Females were reared for 3 days at 30°C prior to egg collection. The MTD-GAL4 driver (G4) is active in the germline at all stages of oogenesis. Representative images of eggshell phenotypes, in order of increasing degree of dorsalization: Wild type; Class I, having a single fused DA and/or ectopic DA material at the base; Class II, having an enlarged dorsal midline and laterally positioned DAs; Class III, having DA material completely surrounding the operculum. Anterior is to the left. Chart shows quantitation of phenotypic classes; N > 270 for each genotype. (b) Broad localizes to nuclei of follicle cells that will form the DA tubes at later stages. Representative images of Broad localization patterns: Wild type; Class I, having a narrow or absent midline; Class II, having an enlarged midline; Class III, having an expanded tube primordium around the circumference of the egg. Anterior is up and to the left. Chart shows quantitation of phenotypic classes; N ≥ 10 for each genotype.