Fig. 9. IF/FISH demonstrates that expression of S. lebanonensis Grk does not mislocalize endogenous gurken protein or transcript.

(a) Two hypotheses predict different localization patterns of endogenous D. melanogaster gurken protein and transcript in the presence of grk^Sl. Left: aberrant eggshells result from mis- localization of endogenous grk RNA or protein due to competition for regulatory factors with the introduced grk^Sl transgene products. Right: aberrant eggshell structures result from high activity of the introduced grk^Sl transgene. (b) Chart shows quantitation of phenotypic classes; N ≥ 10 for each genotype. (c-e) Representative images of phenotypic classes: (c) wild-type localization of grk transcript and Grk protein to the anterodorsal corner of the oocyte; (d) mislocalized and/or high levels of Grk protein, with wild-type grk transcript localization; (e) mislocalized and/or high levels of grk transcript, with wild-type Grk protein; (f) both mislocalized and/or high levels of grk transcript and Grk protein. Images show a lateral orientation with anterior to the left and dorsal up.