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. 2018 Feb 8;8:2624. doi: 10.1038/s41598-018-21084-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo ultrasonography of the implanted tumor in C57BL/6 mice. (A) Shows ultrasonographs captured both before and after contrast administration (i.v.). Time-dependent contrast enhancement was followed till 10 minutes of the contrast administration. (B) Shows the quantification of the relative time-dependent contrast enhancement by both PSPLBC and SonoVue; (*p ≤ 0.05).