Fig. 1.

Pof8 contributes to telomerase-dependent telomere maintenance. a, b Southern blot analysis of indicated strains probed with S. pombe telomere repeats. After strains were generated by transformation or genetic crosses, they were extensively restreaked on YES plates to achieve terminal telomere length. c–e Quantitative real-time PCR ChIP analysis to monitor association with telomeres for (c) Trt1, (d) Est1, and (e) Pof8 for indicated genetic backgrounds. Plots show mean values plus/minus SEM from at least 5 independent experiments. Raw data and statistical analysis are available in Supplementary Data 1. Expression levels of myc-tagged proteins used in ChIP assays were monitored by western blot analysis. Anti-Cdc2 blots served as loading control. Molecular weight (kDa) of size markers are also indicated