Fig. 5.

Over-expression of TER1 is unable to suppress telomerase defect of pof8∆ cells. a Introduction of a nmt1 promoter-controlled TER1 expression plasmid allowed restoration of TER1 RNA levels in pof8∆ cells to ~2-fold or ~3-fold over pof8⁺ wild-type cells when grown in minimum media under repressed (+B1) or induced (−B1) conditions, respectively. TER1 RNA expression levels were normalized to his1⁺ mRNA expression. Error bars correspond to SEM from 3 independent experiments. b Southern blot analysis of telomere length for indicated strains. While TER1 plasmid fully restored wild-type telomere length in ter1∆ cells, it was unable to suppress telomere shortening in pof8∆ cells. (c) Restoration of TER1 RNA expression failed to restore TER1-Trt1 interaction in pof8∆ cells. Plot shows fold enrichment of TER1 RNA recovered after IP compared to no tag control strain. d Restoration of TER1 RNA expression failed to restore Trt1 recruitment at telomeres. Error bars for plots in c and d correspond to SEM from at least 4 independent experiments. Raw data and statistical analysis are available in Supplementary Data 1