Fig. 6.
RFX1 restrains Th17 cell differentiation in vitro. a Representative flow cytometric analysis of Th17 cells polarized from naive T cells of Rfx1f/f and Rfx1f/f-cre mice on days 3, 5, and 7 in the left panel. b shows the quantification (mean ± s.d.; n = 4 mice per group). c–f mRNA expression levels of Th17-related genes, including IL-17A (c), IL-17F (d), IL-23R (e), and Rorc (f) (mean ± s.d.; n = 4). g, h Flow cytometric analysis of in vitro-induced Th17 cells from naive T cells of Rfx1f/f-cre mice transfected with mouse Rfx1 expression vector (Rfx1-over) or empty expression vector (EV) 5 days after transfection. i–k mRNA expression levels of Rfx1 (i), IL-17A (j), and Rorc (k) genes in Th17 cells induced in vitro. Data are representative of three independent experiments (mean ± s.d.; n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; n.s., not significant, two-tailed Student’s t-test