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. 2018 Feb 8;8:2668. doi: 10.1038/s41598-018-20763-w

Figure 4.

Figure 4

Functional changes in the cytoskeletal stiffness by TGFβ1. (a) VSM cells (young and old) were treated for 24 h with or without 5 ng/ml TGFβ1, and changes in cell stiffness were measured by MTC. Data are presented as Geometric Mean ± SE (n = 175–410 individual cell measurements). (b) Old VSM cells were transfected with 25 nM small interference RNA (siRNA) against TGFβ1. After 48 h of transfection, TGFβ1 protein level was determined by western blot (Full gel/blot is shown in the Supplementary Figure 6). (c) Stiffness of old VSM cells transfected with control or siRNA against TGFβ. Data are presented as Geometric Mean ± SE (n = 112–162 individual cell measurements). (d) Stiffness of old VSM cells treated for 24 h with TGFβ1 receptor inhibitors, A8301 and GW788388 (0–10 μM). Data are presented as Geometric Mean ± SE (n = 184–338 individual cell measurements). (e) TGFβ1-induced cell stiffness in young VSM cells treated for 24 h with or without the inhibitors (10 μM A8301 and 10 μM GW788388). DMSO (0.1%) was used as control. Data are presented as Geometric Mean ± SE (n = 439–477 individual cell measurements). *P < 0.05; ****P < 0.0001.