Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 8;9:562. doi: 10.1038/s41467-018-02915-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — Enhanced anticancer capacity of LSC-P and LSC-C nanoparticles under NIR laser irradiation in vitro. a–b Photographs and quantitative data of crystal violet assay of 2D-cultured NCI/RES-ADR multidrug-resistant cells a and CSC-enriched NCI/RES-ADR spheres b after treated with free paclitaxel (PTX), free irinotecan (CPT-11), LSC-C nanoparticles, or LSC-P nanoparticles without or with NIR laser (L) irradiation (LSC-C + L or LSC-P + L, 1 W cm⁻² for 1 min). Error bars represent s.d. (n = 3). c Both 2D-cultured NCI/RES-ADR cells and CSC-enriched NCI/RES-ADR spheres were cultured with empty LSC nanoparticles (0.2 mg ml⁻¹) for 12 h. After laser irradiation (1 W cm⁻² for 1 min), the cells were further cultured with free PTX or CPT-11 dissolved (with the aid of 1% DMSO) in fresh medium for 24 h, showing the free PTX or CPT−11 could induce more cytotoxicity compared with the free drugs treated cells in a–b. This is probably owing to the inhibition of drug resistance ability of the cells after the combined treatment of LSC nanoparticles and laser (LSC + L). Error bars represent s.d. (n = 3). d–e Distribution of PTX-F (PTX labeled with fluorescein isothiocyanate (d) or CPT-11 (e) in free PTX-F or CPT−11, LSC + L treatment with free CPT-11 or PTX-F (LSC + L, + C or LSC + L, + PF), and LSC-C or LSC-PF treated multidrug-resistant cells. The results of both CPT-11 and PTX-F show that more drug could be taken up by the multidrug-resistant cancer cells after treated with LSC + L, which further confirms the drug-resistant capacity of the multidrug-resistant cancer cells can be overcome by treating the cancer cells with LSC nanoparticles and laser. Moreover, the highest drug fluorescence can be seen in multidrug-resistant cells of the LSC-C and LSC-PF groups, indicating more drugs can be delivered into the multidrug-resistant cancer cells by encapsulating the drugs in the LSC nanoparticles. The merged views of the fluorescence of CPT-11 and PTX-F with mitochondria further confirm the capability of the LSC nanoparticles in targeting mitochondria. LSC-P: paclitaxel (PTX) laden LSC nanoparticles and LSC-C: irinotecan (CPT-11) laden LSC nanoparticles. Scale bars: 20 μm