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. 2018 Feb 8;8:2683. doi: 10.1038/s41598-018-21040-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Regulation of the cell cycle by B55α and B55β. (A) Metaphase-arrested Xenopus egg extracts were supplemented with recombinant B55α or control buffer for 10 min. These extracts were then released into interphase by the addition of Calcium at time 0. The extract samples were collected at the indicated time points and analyzed by immunoblotting. Phosphorylation (band-shift) of Cdc27 and Cdc25, and the global phosphorylation of Cdk substrates are markers of mitosis. (B) Interphase Xenopus egg extracts were supplemented with recombinant B55α or control buffer. The extract samples were collected at the indicated time points and analyzed by immunoblotting. Mitosis is indicated by Cdc27 phosphorylation. (C) MBP-B55α and B55β were purified and examined by Coomassie staining. (D) Interphase Xenopus egg extracts were supplemented with recombinant B55α, B55β, or control buffer. The extract samples were collected at the indicated time points and analyzed by immunoblotting. Mitosis is indicated by Cdc27 phosphorylation.