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. 2018 Feb 8;8:2600. doi: 10.1038/s41598-018-20925-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The interface of the predicted PilO_Δ109 dimer is similar to that of EpsM. (A) The dimer interface found in the PilO^2RJZ_Δ109 dimer (violet) (PDB 2RJZ³⁰). (B) The most energetically favourable predicted interaction interface for the PilO_Δ109 structure (grey). (C) The EpsM_Δ65 crystal structure (cyan) from the T2SS of V. cholerae (PDB 1UV7³⁸). Black arrows indicate direction of the N-termini for each subunit.