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. 2018 Feb 8;8:2600. doi: 10.1038/s41598-018-20925-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Highly conserved residues in unstructured regions on the PilO_Δ109 structure probed by site directed mutagenesis. (A) The sequence conservation of the PilO families from a subset of Pseudomonads (P. fluorescens, P. syringae pv. phaseolicola 1448 A, P. savastanoi, P. syringae, P. protegens CHA0, P. avellanae, P. stutzeri A1501, P. resinovorans, P. chlororaphis) are indicated by the bars. The conservation of the residues is indicated by the bar and the intensity of the color (high conservation, high bar and bright yellow; low conservation, low bar and a dark brown color). The α-helices (red rectangles) and β-strands (green arrows) indicate secondary structure elements present in the PilO_Δ109 structure. (B) The structure of PilO_Δ109 indicating the position of the residues chosen for site directed mutagenesis. Conserved and unstructured residues (orange), β-strand breakers (blue), and the position of the di-methyl Lys (green), are indicated.