Table 2.
Summary of PilO mutants and their phenotypes.
| PilO | Location | Twitching motility | Surface pili |
|---|---|---|---|
| LL132-133AA | Region 1 – β1 | Yes | Yes |
| PE134-135AL | Region 1 – β1 | Yes | Yes |
| F140A | Region 1 – β2–β3 | Yes | Yes |
| Y141A | Region 1 – β2-β3 | Yes | Yes |
| L167A | Region 2 – α2-β4 | Yes | Yes |
| P168A | Region 2 – α2-β4 | Yes | Yes |
| R169D | Region 2 – α2-β4 | Reduced (≈ 40%) | Reduced (≈ 65%) |
| I170A | Region 2 – α2-β4 | Reduced (≈ 50%) | Reduced (≈ 70%) |
| V171A | Region 2 – α2-β4 | Yes | Yes |
| TL172-173AA | Region 2 – β4 | Yes | Yes |
| H174A | Region 2 – β4 | Yes | Yes |
| D175R | Region 2 – β4-β5 | Yes | Yes |
| F176A | Region 2 – β4-β5 | Yes | Yes |